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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
14/01/2011 |
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Data da última atualização: |
20/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTENARO-DA-SILVA, D.; PASSAIA, G.; HENRIQUES, J. A. P.; MARGIS, R.; PASQUALI, G.; REVERS, L. F. |
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Afiliação: |
DANIELLE COSTENARO-DA-SILVA, UFRGS; GISELE PASSAIA, CNPUV (bolsista); JOÃO A. P. HENRIQUES, UFRGS; ROGÉRIO MARGIS, UFRGS; GIANCARLO PASQUALI, UFRGS; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Plant Science, Limerick, v. 179, n. 5, p. 510-519, nov. 2010. |
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Volume: |
179 |
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Páginas: |
510-519 |
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Idioma: |
Inglês |
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Conteúdo: |
Sultanine grapevine (Vitis vinifera L.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. Despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. Actually, studies concerning berry development in seedless grapes are scarce at the molecular level. We therefore developed a representational difference analysis (RDA) modified method named Bulk Representational Analysis of Transcripts (BRAT) in the attempt to identify genes specifically associated with each of the main developmental stages of Sultanine grapevine berries. A total of 2400 transcript-derived fragments (TDFs) were identified and cloned by RDA according to three specific developmental berry stages. After sequencing and in silico analysis, 1554 (64.75%) TDFs were validated according to our sequence quality cut-off. The assembly of these expressed sequence tags (ESTs) yielded 504 singletons and 77 clusters, with an overall EST redundancy of approximately 67%. Amongst all stage-specific cDNAs, nine candidate genes were selected and, along with two reference genes, submitted to a deeper analysis of their temporal expression profiles by reverse transcription-quantitative PCR. Seven out of nine genes proved to be in agreement with the stage-specific expression that allowed their isolation by RDA. |
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Palavras-Chave: |
Desenvolvimento da baga; Expressão gênica; Identificação genética; PCR em tempo real; RDA; Uva de mesa; Uva sem semente. |
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Thesagro: |
Biologia molecular; Genética; Uva; Viticultura. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25458/1/PlantScience-Revers-2010.pdf
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Marc: |
LEADER 02505naa a2200337 a 4500
001 1873059
005 2019-05-20
008 2010 bl uuuu u00u1 u #d
100 1 $aCOSTENARO-DA-SILVA, D.
245 $aIdentification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine.$h[electronic resource]
260 $c2010
300 $a510-519 179
490 $v179
520 $aSultanine grapevine (Vitis vinifera L.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. Despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. Actually, studies concerning berry development in seedless grapes are scarce at the molecular level. We therefore developed a representational difference analysis (RDA) modified method named Bulk Representational Analysis of Transcripts (BRAT) in the attempt to identify genes specifically associated with each of the main developmental stages of Sultanine grapevine berries. A total of 2400 transcript-derived fragments (TDFs) were identified and cloned by RDA according to three specific developmental berry stages. After sequencing and in silico analysis, 1554 (64.75%) TDFs were validated according to our sequence quality cut-off. The assembly of these expressed sequence tags (ESTs) yielded 504 singletons and 77 clusters, with an overall EST redundancy of approximately 67%. Amongst all stage-specific cDNAs, nine candidate genes were selected and, along with two reference genes, submitted to a deeper analysis of their temporal expression profiles by reverse transcription-quantitative PCR. Seven out of nine genes proved to be in agreement with the stage-specific expression that allowed their isolation by RDA.
650 $aBiologia molecular
650 $aGenética
650 $aUva
650 $aViticultura
653 $aDesenvolvimento da baga
653 $aExpressão gênica
653 $aIdentificação genética
653 $aPCR em tempo real
653 $aRDA
653 $aUva de mesa
653 $aUva sem semente
700 1 $aPASSAIA, G.
700 1 $aHENRIQUES, J. A. P.
700 1 $aMARGIS, R.
700 1 $aPASQUALI, G.
700 1 $aREVERS, L. F.
773 $tPlant Science, Limerick$gv. 179, n. 5, p. 510-519, nov. 2010.
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Embrapa Uva e Vinho (CNPUV) |
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